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E, C431S is the lone free cysteine mutation to specifically inhibit Ub-VS conjugation. F, E3 activity of Parkin lacking the Ubl and RING1 domains in cells. HeLa ...
Parkin has been reported to generate poly-Ubiquitin chains in K6, K11, K48, and K63 linkages both in vitro and in vivo. This recombinant protein is untagged, ...
Autosomal recessive mutations in the Parkin gene cause Parkinson's disease. Parkin encodes an ubiquitin E3 ligase that functions together with the kinase PINK1 ...
Mutating the putative catalytic cysteine to serine (Parkin C431S) ... We found that Parkin can bind to K63-linked ubiquitin chains, and that targeting K63- ...
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Plasmid pGEX-parkin C431S from Dr. Kalle Gehring's lab contains the insert Parkin C431S and is published in Science. 2013 May 9. This plasmid is available ...
May 14, 2013 · Mutating the putative catalytic cysteine to serine (Parkin C431S) traps ubiquitin, and surprisingly, also abrogates Parkin mitochondrial ...
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Parkin C431S ; Expression. bacteria ; Terminus and Tag. Uncleaved ; Protein Subtype. Ubiquitin ; DU Number. DU39784 ; Unit Source. Protein Phosphorylation and ...
PARKIN phosphorylation on S65 and activation as measured by oxy-ester formation with PARKINC431S (3, 12, 18, 19) can occur in the absence of PARKIN ...
Jul 19, 2023 · This effect was independent of mitochondrial and lysosomal density, which was similar between parkin WT and parkin C431S-expressing cells (fig.
Aug 25, 2023 · Expression of the Parkin C431S mutant, which lacks E3 ligase activity, in PINK1 WT or KO cells did not alter ER calcium release or cytosolic ...