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  1. pubmed.ncbi.nlm.nih.gov › 17446171Electron transport pathway for a Streptomyces ... - PubMed

    Jun 15, 2007 · Relatively few electron transport pathways to P450s have been characterized in bacteria, particularly streptomycete species. One of the 18 P450s in Streptomyces coelicolor A3(2), P450 105D5, was found to bind fatty acids tightly and form hydroxylated products when electrons were delivered from heterologous systems.

    • Young-Jin Chun, Young-Jin Chun, Tsutomu Shimada, Raymundo Sanchez-Ponce, Martha V. Martin, Li Lei, B...
    • 2007

  2. www.sciencedirect.com › science › articleElectron Transport Pathway for a Streptomyces ... - ScienceDirect

    Jun 15, 2007 · The six ferredoxin (Fdx) and four flavoprotein Fdx reductase (FDR) proteins coded by genes in S. coelicolor were expressed in Escherichia coli, purified, and used to characterize the electron transfer pathway. Of the many possibilities, the primary pathway was NADH → FDR1 → Fdx4 → P450 105D5.

    • Young-Jin Chun, Young-Jin Chun, Tsutomu Shimada, Raymundo Sanchez-Ponce, Martha V. Martin, Li Lei, B...
    • 2007

  3. www.researchgate.net › figure › Proposed-catalyticProposed catalytic cycle for P450 105D5. The scheme is based ...

    P450 105D5 is the product of one of the 18 P450 genes in S. coelicolor A3 (2) and catalyzes the hydroxylation of fatty acids, which are endogenous compounds in this bacterium.

  4. www.jbc.org › article › S0021-9258(20)69051-2ElectronTransportPathwayfora Streptomyces CytochromeP450

    Streptomyces coelicolor A3(2), P450 105D5, was found to bind fatty acids tightly and form hydroxylated products when elec-trons were delivered from heterologous systems. The six ferre-doxin(Fdx)andfourflavoproteinFdxreductase(FDR)proteins coded by genes in S. coelicolor were expressed in Escherichia

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  6. www.ncbi.nlm.nih.gov › pmc › articlesStreptomyces Cytochrome P450 Enzymes and Their Roles in the ...

    Dec 18, 2018 · Electron transport pathway for a Streptomyces cytochrome P450: cytochrome P450 105D5-catalyzed fatty acid hydroxylation in Streptomyces coelicolor A3(2) J. Biol. Chem. 2007; 282:17486–17500. doi: 10.1074/jbc.M700863200.

    • Myung A Cho, Songhee Han, Young Ran Lim, Vitchan Kim, Harim Kim, Donghak Kim
    • 10.4062/biomolther.2018.183
    • 2019
    • 2019/02

  7. www.jbc.org › cms › 10Electron Transport Pathway for a Streptomyces Cytochrome P450

    Spectral changes induced by binding of lauric acid to P450 105D5. Varying concentrations of lauric acid (dissolved in CH 3 OH) were added to a solution of P450 105D5 (1.0 µM) in 0.10 M potassium phosphate buffer (pH 7.4) containing 20% glycerol (v/v). A, Difference spectra, with the starting spectrum subtracted from the subsequent traces. The

  8. www.researchgate.net › publication › 6382984(PDF) Electron transport pathway for a Streptomyces ...

    Jun 1, 2007 · Of the many possibilities, the primary pathway was NADH --> FDR1 --> Fdx4 --> P450 105D5. The genes coding for FDR1, Fdx4, and P450 105D5 are located close together in the S. coelicolor genome.